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However, there is no single effective recovery aid available for the rehabilitation of patients after stroke ( Lindau et al., 2014). This disability is primarily attributed to the brain lesion, which may result in permanent neurological deficits. On average, every 40 s someone in the United States has a stroke ( Scherbakov et al., 2013 Benjamin et al., 2017).

In conclusion, EA enhances neurobehavioral functional recovery against ischemic stroke through targeting of SOX2-mediated axonal regeneration by miR-132.Ī leading cause of disability in western countries is stroke, which has high lethality. Moreover, our results also suggested that up-regulated miR-132 suppressed SOX2 in primary neurons after oxygen-glucose deprivation (OGD), which promoted neurite outgrowth.

The results showed that EA up-regulated miR-132 thus suppressing SOX2 expression in vivo after MCAO, which obviously ameliorated neurobehavioral functional recovery. This study aimed to identify whether up-regulation of miR-132 by EA improved the damaged nerves after stroke and to screen the potential target of miR-132. Our previous studies also have shown that the expression of miRNA-132 (miR-132) is obviously down-regulated after stroke by middle cerebral artery occlusion (MCAO), which can be up-regulated by EA. During cerebral ischemia reperfusion, miRNAs perform numerous biological functions in the central nervous system related to regeneration and repair of damaged nerves. MicroRNAs (miRNAs) are abundant in the brain and are involved in post-transcriptional gene regulation. Our previous studies have shown that electroacupuncture (EA) enhances neurobehavioral functional recovery after ischemic stroke, however, the underlying regulatory mechanisms remain unclear.

5Department of Anesthesiology, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, China.

4Department of Anesthesiology, The Northwest Women’s and Children’s Hospital, Xi’an, China.

3Department of Anesthesiology, The Affiliated Hospital of Yan’an University, Yan’an, China.

2Department of Anesthesiology, Second Hospital of Shanxi Medical University, Taiyuan, China.

1Department of Anesthesiology and Perioperative Medicine, Xijing Hospital, Air Force Medical University, Xi’an, China.

Xiaoying Zhao 1,2†, Fuhai Bai 1†, Erfei Zhang 1,3, Dandan Zhou 1,4, Tao Jiang 5, Heng Zhou 1 and Qiang Wang 1,5*

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